abstract：:To assess the genetic consequences of induced pluripotent stem cell (iPSC) reprogramming, we sequenced the genomes of ten murine iPSC clones derived from three independent reprogramming experiments, and compared them to their parental cell genomes. We detected hundreds of single nucleotide variants (SNVs) in every clo...

journal_title：Cell stem cell

authors： Young MA,Larson DE,Sun CW,George DR,Ding L,Miller CA,Lin L,Pawlik KM,Chen K,Fan X,Schmidt H,Kalicki-Veizer J,Cook LL,Swift GW,Demeter RT,Wendl MC,Sands MS,Mardis ER,Wilson RK,Townes TM,Ley TJ

更新日期：2012-05-04 00:00:00

abstract：:Although distinct human induced pluripotent stem cell (hiPSC) lines can display considerable epigenetic variation, it has been unclear whether such variability impacts their utility for disease modeling. Here, we show that although low-passage female hiPSCs retain the inactive X chromosome of the somatic cell they are...

journal_title：Cell stem cell

authors： Mekhoubad S,Bock C,de Boer AS,Kiskinis E,Meissner A,Eggan K

更新日期：2012-05-04 00:00:00

abstract：:Achieving immune tolerance through cell transplantation is a promising approach for treating autoimmune disease. In this issue of Cell Stem Cell, Akiyama et al. (2012) demonstrate that human and mouse mesenchymal stem cells can induce immune suppression by attracting and killing autoreactive T cells, which stimulates ...

journal_title：Cell stem cell

authors： Murphy WJ,Nolta JA

更新日期：2012-05-04 00:00:00

abstract：:How DNA methylation inhibitors exert their anticancer effects in patients is not well understood. In the latest issue of Cancer Cell, Tsai et al. (2012) use low-dose drug treatment to induce persistent attenuation of tumorigenicity by targeting tumor-initiating cells. ...

journal_title：Cell stem cell

authors： Shen H,Laird PW

更新日期：2012-04-06 00:00:00

abstract：:The utility of induced pluripotent stem cells (iPSCs) as models to study diseases and as sources for cell therapy depends on the integrity of their genomes. Despite recent publications of DNA sequence variations in the iPSCs, the true scope of such changes for the entire genome is not clear. Here we report the whole-g...

journal_title：Cell stem cell

authors： Cheng L,Hansen NF,Zhao L,Du Y,Zou C,Donovan FX,Chou BK,Zhou G,Li S,Dowey SN,Ye Z,NISC Comparative Sequencing Program.,Chandrasekharappa SC,Yang H,Mullikin JC,Liu PP

更新日期：2012-03-02 00:00:00

abstract：:A crucial limitation to our understanding of Alzheimer's disease has been the inability to test hypotheses on live, patient-specific neurons. A recent study in Nature by Israel et al. (2012) reports that iPSC-derived neurons from AD patients recapitulate multiple aspects of disease pathology. ...

journal_title：Cell stem cell

authors： Choi SH,Tanzi RE

更新日期：2012-03-02 00:00:00

abstract：:Increasing evidence suggests tumors are maintained by cancer stem cells; however, their nature remains controversial. In a HoxA9-Meis1 (H9M) model of acute myeloid leukemia (AML), we found that tumor-initiating activity existed in three, immunophenotypically distinct compartments, corresponding to disparate lineages o...

journal_title：Cell stem cell

authors： Gibbs KD Jr,Jager A,Crespo O,Goltsev Y,Trejo A,Richard CE,Nolan GP

更新日期：2012-02-03 00:00:00

abstract：:In this issue of Cell Stem Cell, Csaszar et al. (2012) develop a culture method that overcomes current limitations in ex vivo hematopoietic stem/progenitor cell expansion by continuously diluting inhibitory signaling factors and maintaining stem cell density. This approach enhances the generation of precursors with po...

journal_title：Cell stem cell

authors： Bernstein ID,Delaney C

更新日期：2012-02-03 00:00:00

abstract：:Stem cells have the capacity to both self-renew and generate postmitotic cells. Long-term tracking of individual clones in their natural environment constitutes the ultimate way to validate postembryonic stem cells. We identify retinal stem cells (RSCs) using the spatiotemporal organization of the fish retina and foll...

journal_title：Cell stem cell

authors： Centanin L,Hoeckendorf B,Wittbrodt J

更新日期：2011-12-02 00:00:00

abstract：:Regulation of hematopoietic stem cell (HSC) dormancy by specific cell types in the hematopoietic niche remains poorly understood. Yamazaki et al. (2011) now report that nerve-associated nonmyelinating Schwann cells activate TGF-β to maintain dormant HSCs, suggesting that glia are novel players in the bone marrow niche...

journal_title：Cell stem cell

authors： Brückner K

更新日期：2011-12-02 00:00:00

abstract：:Nodal and Activin belong to the TGF-β superfamily and are important regulators of embryonic stem cell fate. Here we investigated whether Nodal and Activin regulate self-renewal of pancreatic cancer stem cells. Nodal and Activin were hardly detectable in more differentiated pancreatic cancer cells, while cancer stem ce...

journal_title：Cell stem cell

authors： Lonardo E,Hermann PC,Mueller MT,Huber S,Balic A,Miranda-Lorenzo I,Zagorac S,Alcala S,Rodriguez-Arabaolaza I,Ramirez JC,Torres-Ruíz R,Garcia E,Hidalgo M,Cebrián DÁ,Heuchel R,Löhr M,Berger F,Bartenstein P,Aicher A,Hee

更新日期：2011-11-04 00:00:00

abstract：:Tissue homeostasis over the life of an organism relies on both self-renewal and multipotent differentiation of stem cells. Hematopoietic stem cells (HSCs) reside in a hypoxic bone marrow environment, and their metabolic status is distinct from that of their differentiated progeny. HSCs generate energy mainly via anaer...

journal_title：Cell stem cell

authors： Suda T,Takubo K,Semenza GL

更新日期：2011-10-04 00:00:00

abstract：:Cell cycle regulators play critical roles in the balance between hematopoietic stem cell (HSC) dormancy and proliferation. In this study, we report that cell cycle entry proceeded normally in HSCs null for cyclin-dependent kinase (CDK) inhibitor p57 due to compensatory upregulation of p27. HSCs null for both p57 and p...

journal_title：Cell stem cell

authors： Zou P,Yoshihara H,Hosokawa K,Tai I,Shinmyozu K,Tsukahara F,Maru Y,Nakayama K,Nakayama KI,Suda T

更新日期：2011-09-02 00:00:00

abstract：:Erythropoietin, the red blood cell-making cytokine, is also a potential cytoprotective agent in heart disease. In this issue of Cell Stem Cell, Hoch et al. (2011) use two heart failure models, including chemotherapeutic cardiotoxicity, to reveal a mechanistic connection between reduced cardiomyocyte production of eryt...

journal_title：Cell stem cell

authors： Schneider MD

更新日期：2011-08-05 00:00:00

abstract：:Adipose stromal cells (ASCs) serve as mesenchymal progenitors in white adipose tissue (WAT). Intercellular interactions involving ASCs have remained obscure. By merging phage display technology with fluorescence-activated cell sorting (FACS), we screened a combinatorial library for peptides that target mouse ASCs in v...

journal_title：Cell stem cell

authors： Daquinag AC,Zhang Y,Amaya-Manzanares F,Simmons PJ,Kolonin MG

更新日期：2011-07-08 00:00:00

abstract：:Human induced pluripotent stem cells (HiPSCs) appear to be highly similar to human embryonic stem cells (HESCs). Using two genetic lineage-tracing systems, we demonstrate the generation of iPSC lines from human pancreatic islet beta cells. These reprogrammed cells acquired markers of pluripotent cells and differentiat...

journal_title：Cell stem cell

authors： Bar-Nur O,Russ HA,Efrat S,Benvenisty N

更新日期：2011-07-08 00:00:00

abstract：:Combination of stem cell-based approaches with gene-editing technologies represents an attractive strategy for studying human disease and developing therapies. However, gene-editing methodologies described to date for human cells suffer from technical limitations including limited target gene size, low targeting effic...

journal_title：Cell stem cell

authors： Liu GH,Suzuki K,Qu J,Sancho-Martinez I,Yi F,Li M,Kumar S,Nivet E,Kim J,Soligalla RD,Dubova I,Goebl A,Plongthongkum N,Fung HL,Zhang K,Loring JF,Laurent LC,Izpisua Belmonte JC

更新日期：2011-06-03 00:00:00

abstract：:Bmi1 is required for efficient self-renewal of hematopoietic stem cells (HSCs) and leukemic stem cells (LSCs). In this study, we investigated whether leukemia-associated fusion proteins, which differ in their ability to activate Hox expression, could initiate leukemia in the absence of Bmi1. AML1-ETO and PLZF-RARα, wh...

journal_title：Cell stem cell

authors： Smith LL,Yeung J,Zeisig BB,Popov N,Huijbers I,Barnes J,Wilson AJ,Taskesen E,Delwel R,Gil J,Van Lohuizen M,So CW

更新日期：2011-06-03 00:00:00

abstract：:The Notch signaling pathway is activated in the majority of T cell acute lymphoblastic leukemias (T-ALL). Adding to the complexity of Notch signaling in hematopoiesis, recently in Nature, Klinakis et al. (2011) demonstrate a tumor-suppressor function for the Notch pathway in myeloid malignancy. ...

journal_title：Cell stem cell

authors： Walkley CR,Izon DJ,Purton LE

更新日期：2011-06-03 00:00:00

abstract：:Production of new neurons in the adult hippocampus decreases with age; this decline may underlie age-related cognitive impairment. Here we show that continuous depletion of the neural stem cell pool, as a consequence of their division, may contribute to the age-related decrease in hippocampal neurogenesis. Our results...

journal_title：Cell stem cell

authors： Encinas JM,Michurina TV,Peunova N,Park JH,Tordo J,Peterson DA,Fishell G,Koulakov A,Enikolopov G

更新日期：2011-05-06 00:00:00

abstract：:Understanding the basis of the unrestricted multilineage differentiation potential of pluripotent cells will be of developmental and translational consequence. We propose that pluripotency transcription factors are lineage specifiers that direct commitment to specific fetal lineages. Individual factors bestow the abil...

journal_title：Cell stem cell

authors： Loh KM,Lim B

更新日期：2011-04-08 00:00:00

abstract：:Embryonic stem cells (ESCs) are an attractive source for tissue regeneration and repair therapies because they can be differentiated into virtually any cell type in the adult body. However, for this approach to succeed, the transplanted ESCs must survive long enough to generate a therapeutic benefit. A major obstacle ...

journal_title：Cell stem cell

authors： Pearl JI,Lee AS,Leveson-Gower DB,Sun N,Ghosh Z,Lan F,Ransohoff J,Negrin RS,Davis MM,Wu JC

更新日期：2011-03-04 00:00:00

abstract：:To date, pluripotent epiblast stem cells (EpiSCs) had only been derived from postimplantation mouse embryos. In this issue of Cell Stem Cell, Najm et al. (2011) demonstrate that EpiSCs can be routinely derived from preimplantation embryos, showing that both human and mouse blastocysts can produce the same class of pri...

journal_title：Cell stem cell

authors： Kunath T

更新日期：2011-03-04 00:00:00

abstract：:A survey of U.S. stem cell scientists shows that uncertainty following the legal challenge to the Obama Administration's hESC research policy has negative scientific and economic impacts and affects a range of stem cell scientists, not just those working with hESCs. The international implications of these results are ...

journal_title：Cell stem cell

authors： Levine AD

更新日期：2011-02-04 00:00:00

abstract：:Recent studies have shown that tissue-specific stem cells (SCs) found throughout the body respond differentially to DNA damage. In this review, we will discuss how different SC populations sense and functionally respond to DNA damage, identify various common and distinct mechanisms utilized by tissue-specific SCs to a...

journal_title：Cell stem cell

authors： Blanpain C,Mohrin M,Sotiropoulou PA,Passegué E

更新日期：2011-01-07 00:00:00

abstract：:Steroid hormones are known systemic regulators of multiple normal and cancerous tissues; however, whether or how they impact the fate and function of adult stem cells is unclear. In the Drosophila ovary, insulin signals modulate the proliferation and self-renewal of germline stem cells (GSCs), yet despite evidence tha...

journal_title：Cell stem cell

authors： Ables ET,Drummond-Barbosa D

更新日期：2010-11-05 00:00:00

abstract：:The 2010 Annual Meeting of the International Society for Stem Cell Research (ISSCR) was held in San Francisco in June with an exciting program covering a wealth of stem cell research from basic science to clinical research. ...

journal_title：Cell stem cell

authors： Elefanty AG,Blelloch R,Passegué E,Wernig M,Mummery CL

更新日期：2010-10-08 00:00:00

abstract：:The generation of induced pluripotent stem cells (iPSCs) provides a novel method to facilitate investigations into the mechanisms that control stem cell pluripotency and self-renewal. Myc has previously been shown to be critical for murine embryonic stem cell (mESC) maintenance, while also enhancing directed reprogram...

journal_title：Cell stem cell

authors： Smith KN,Singh AM,Dalton S

更新日期：2010-09-03 00:00:00

abstract：:Knowledge of both the global chromatin structure and the gene expression programs of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) should provide a robust means to assess whether the genomes of these cells have similar pluripotent states. Recent studies have suggested that ESCs and iPSCs...

journal_title：Cell stem cell

authors： Guenther MG,Frampton GM,Soldner F,Hockemeyer D,Mitalipova M,Jaenisch R,Young RA

更新日期：2010-08-06 00:00:00

abstract：:Neural precursor cells (NPCs) reside in the subventricular zone in association with blood vessels and ependymal cells. In this issue of Cell Stem Cell, Kokovay et al. (2010) show that SDF1 directs the association of NPCs with this niche and regulates their lineage progression in a stage-specific manner. ...

journal_title：Cell stem cell

authors： Siegenthaler JA,Pleasure SJ

更新日期：2010-08-06 00:00:00

abstract：:Epithelial-to-mesenchymal transition (EMT) is a developmental process important for cell fate determination. Fibroblasts, a product of EMT, can be reset into induced pluripotent stem cells (iPSCs) via exogenous transcription factors but the underlying mechanism is unclear. Here we show that the generation of iPSCs fro...

journal_title：Cell stem cell

authors： Li R,Liang J,Ni S,Zhou T,Qing X,Li H,He W,Chen J,Li F,Zhuang Q,Qin B,Xu J,Li W,Yang J,Gan Y,Qin D,Feng S,Song H,Yang D,Zhang B,Zeng L,Lai L,Esteban MA,Pei D

更新日期：2010-07-02 00:00:00

abstract：:Two independent studies by Li et al. (2010) and Samavarchi-Tehrani et al. (2010) in this issue of Cell Stem Cell suggest that a mesenchymal-to-epithelial transition is a critical initiating event during the derivation of induced pluripotent stem cells (iPSCs) from fibroblasts, indicating remarkable similarities betwee...

journal_title：Cell stem cell

authors： Polo JM,Hochedlinger K

更新日期：2010-07-02 00:00:00

abstract：:To prevent insertional mutagenesis arising from retroviral reactivation, cells of embryonic origin possess a unique capacity to silence retroviruses. Given the distinct modes of X chromosome inactivation between embryonic and extraembryonic lineages, we investigated paradigms of viral extinction. We show that trophect...

journal_title：Cell stem cell

authors： Golding MC,Zhang L,Mann MR

更新日期：2010-05-07 00:00:00

abstract：:Newborn neurons in the adult dentate gyrus pass through several distinct precursor and progenitor classes prior to differentiation. In this issue of Cell Stem Cell, Lugert et al. (2010) characterized their strikingly different proliferative behaviors after neurogenic stimuli or aging. ...

journal_title：Cell stem cell

authors： Breunig JJ,Rakic P

更新日期：2010-05-07 00:00:00

abstract：:The lineage restriction of prospectively isolated hematopoietic progenitors has been traditionally assessed by bulk in vitro culture and transplantation of large number of cells in vivo. These methods, however, cannot distinguish between homogenous multipotent or heterogeneous lineage-restricted populations. Using clo...

journal_title：Cell stem cell

authors： Franco CB,Chen CC,Drukker M,Weissman IL,Galli SJ

更新日期：2010-04-02 00:00:00

abstract：:Similarities between basal cell carcinoma (BCC) tumor cells and hair follicle keratinocytes had previously suggested that BCC originates within the hair follicle bulge stem cell niche. However, in the current Nature Cell Biology, Youssef et al. (2010) show that BCC instead originates in the interfollicular epidermis. ...

journal_title：Cell stem cell

authors： Ridky TW,Khavari PA

更新日期：2010-04-02 00:00:00

abstract：:Polycomb group (PcG) proteins are essential regulators of stem cells. PcG and trithorax group proteins mark developmental regulator gene promoters with bivalent domains consisting of overlapping repressive and activating histone modifications to keep them poised for activation in embryonic stem cells. Bmi1, a componen...

journal_title：Cell stem cell

authors： Oguro H,Yuan J,Ichikawa H,Ikawa T,Yamazaki S,Kawamoto H,Nakauchi H,Iwama A

更新日期：2010-03-05 00:00:00

abstract：:Establishing tissue-specific adult stem cells during organogenesis is important for maintenance of tissue homeostasis throughout the lifetime of the organism. In a recent study in Science, Mathur et al. (2010) describe how progenitor cells in the Drosophila larval midgut create a temporary niche to maintain stem cell ...

journal_title：Cell stem cell

authors： Issigonis M,Matunis E

更新日期：2010-03-05 00:00:00

abstract：:In a recent issue of Nature, Mayack et al. (2010) use young and old parabiotic mice to show that systemic signals from the young parabiont rejuvenate hematopoietic stem cell function via the microenvironmental niche. ...

journal_title：Cell stem cell

authors： Oakley EJ,Van Zant G

更新日期：2010-02-05 00:00:00

abstract：:The study of gastric epithelial homeostasis and cancer has been hampered by the lack of stem cell markers and in vitro culture methods. The Wnt target gene Lgr5 marks stem cells in the small intestine, colon, and hair follicle. Here, we investigated Lgr5 expression in the stomach and assessed the stem cell potential o...

journal_title：Cell stem cell

authors： Barker N,Huch M,Kujala P,van de Wetering M,Snippert HJ,van Es JH,Sato T,Stange DE,Begthel H,van den Born M,Danenberg E,van den Brink S,Korving J,Abo A,Peters PJ,Wright N,Poulsom R,Clevers H

更新日期：2010-01-08 00:00:00